Cloned (Comment) | Organism |
---|---|
gene mtlD or ACIAD1672, DNA and amino acid sequence determination and analysis, real-time-PCR expression analysis, expression of His-tagged enzyme | Acinetobacter baylyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.6 | - |
D-fructose 6-phosphate | pH 7.0, 30°C | Acinetobacter baylyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | the enzyme is strictly salt-dependent and salt-induced, overview | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81300 | - |
x * 81300, about, sequence calculation | Acinetobacter baylyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + NADH + H+ | Acinetobacter baylyi | - |
D-mannitol 1-phosphate + NAD+ | - |
r | |
D-fructose 6-phosphate + NADPH + H+ | Acinetobacter baylyi | - |
D-mannitol 1-phosphate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
gene mtlD or ACIAD1672 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MtlD by nickel affinity chromatography | Acinetobacter baylyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
D-fructose 6-phosphate reduction with NADPH, pH 7.0, 30°C | Acinetobacter baylyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 6-phosphate + NADH + H+ | - |
Acinetobacter baylyi | D-mannitol 1-phosphate + NAD+ | - |
r | |
D-fructose 6-phosphate + NADPH + H+ | - |
Acinetobacter baylyi | D-mannitol 1-phosphate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 81300, about, sequence calculation | Acinetobacter baylyi |
Synonyms | Comment | Organism |
---|---|---|
mannitol-1-phosphate dehydrogenase | - |
Acinetobacter baylyi |
MtlD | - |
Acinetobacter baylyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Acinetobacter baylyi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | 70% of maximal activity at 15°C or 35°C, and 50% at 10°C or 40°C | Acinetobacter baylyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Acinetobacter baylyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 65% and 50% of maximal activity, respectively | Acinetobacter baylyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme prefers NADPH over NADH | Acinetobacter baylyi | |
NAD+ | - |
Acinetobacter baylyi | |
NADH | maximal specific activity with NADH is 15fold lower compared to NADPH | Acinetobacter baylyi | |
NADPH | preferred cofactor | Acinetobacter baylyi |
Organism | Comment | Expression |
---|---|---|
Acinetobacter baylyi | the enzyme is salt-dependent and salt-induced, overview | up |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of gene mtlD results in a complete loss of salt-dependent mannitol biosynthesis | Acinetobacter baylyi |
metabolism | mannitol-1-phosphate dehydrogenase mediates the first step of two-step pathway for osmo-induced synthesis of mannitol, regulated by salinity on the transcriptional as well as on the activity level, overview | Acinetobacter baylyi |